6Z6M

Cryo-EM structure of human 80S ribosomes bound to EBP1, eEF2 and SERBP1

これはPDB形式変換不可エントリーです。

6Z6M の概要

• エントリーDOI: 10.2210/pdb6z6m/pdb
• EMDBエントリー: 11099
• 分子名称: 28S rRNA, 60S ribosomal protein L7a, 60S ribosomal protein L9, ... (86 entities in total)
• 機能のキーワード: ribosome, hibernation
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 84
• 化学式量合計: 4023533.23

構造登録者

Wells, J.N.,Buschauer, R.,Mackens-Kiani, T.,Best, K.,Kratzat, H.,Berninghausen, O.,Becker, T.,Cheng, J.,Beckmann, R. (登録日: 2020-05-28, 公開日: 2020-07-29, 最終更新日: 2024-05-22)

主引用文献

Wells, J.N.,Buschauer, R.,Mackens-Kiani, T.,Best, K.,Kratzat, H.,Berninghausen, O.,Becker, T.,Gilbert, W.,Cheng, J.,Beckmann, R.
Structure and function of yeast Lso2 and human CCDC124 bound to hibernating ribosomes.
Plos Biol., 18:e3000780-e3000780, 2020

PubMed Abstract: Cells adjust to nutrient deprivation by reversible translational shutdown. This is accompanied by maintaining inactive ribosomes in a hibernation state, in which they are bound by proteins with inhibitory and protective functions. In eukaryotes, such a function was attributed to suppressor of target of Myb protein 1 (Stm1; SERPINE1 mRNA-binding protein 1 [SERBP1] in mammals), and recently, late-annotated short open reading frame 2 (Lso2; coiled-coil domain containing short open reading frame 124 [CCDC124] in mammals) was found to be involved in translational recovery after starvation from stationary phase. Here, we present cryo-electron microscopy (cryo-EM) structures of translationally inactive yeast and human ribosomes. We found Lso2/CCDC124 accumulating on idle ribosomes in the nonrotated state, in contrast to Stm1/SERBP1-bound ribosomes, which display a rotated state. Lso2/CCDC124 bridges the decoding sites of the small with the GTPase activating center (GAC) of the large subunit. This position allows accommodation of the duplication of multilocus region 34 protein (Dom34)-dependent ribosome recycling system, which splits Lso2-containing, but not Stm1-containing, ribosomes. We propose a model in which Lso2 facilitates rapid translation reactivation by stabilizing the recycling-competent state of inactive ribosomes.

PubMed: 32687489
DOI: 10.1371/journal.pbio.3000780

実験手法

ELECTRON MICROSCOPY (3.1 Å)