6Z5H

Crystal structure of Aeromonas exotoxin A

これはPDB形式変換不可エントリーです。

6Z5H の概要

• エントリーDOI: 10.2210/pdb6z5h/pdb
• 分子名称: Exotoxin, SODIUM ION, DI(HYDROXYETHYL)ETHER, ... (5 entities in total)
• 機能のキーワード: adp-ribosyltransferase, bacterial toxin, exotoxin, aeromonas, toxin
• 由来する生物種: Aeromonas hydrophila
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 136710.80

構造登録者

Masuyer, G. (登録日: 2020-05-26, 公開日: 2020-06-24, 最終更新日: 2024-11-20)

主引用文献

Masuyer, G.
Crystal Structure of Exotoxin A from Aeromonas Pathogenic Species.
Toxins, 12:-, 2020

PubMed Abstract: exotoxin A (AE) is a bacterial virulence factor recently discovered in a clinical case of necrotising fasciitis caused by the flesh-eating . Here, database mining shows that AE is present in the genome of several emerging pathogenic species. The X-ray crystal structure of AE was solved at 2.3 Å and presents all the hallmarks common to diphthamide-specific mono-ADP-ribosylating toxins, suggesting AE is a fourth member of this family alongside the diphtheria toxin, exotoxin A and cholix. Structural homology indicates AE may use a similar mechanism of cytotoxicity that targets eukaryotic elongation factor 2 and thus inhibition of protein synthesis. The structure of AE also highlights unique features including a metal binding site, and a negatively charged cleft that could play a role in interdomain interactions and may affect toxicity. This study raises new opportunities to engineer alternative toxin-based molecules with pharmaceutical potential.

PubMed: 32549399
DOI: 10.3390/toxins12060397

実験手法

X-RAY DIFFRACTION (2.3 Å)