6Z3Z

CryoEM structure of horse sodium/proton exchanger NHE9 without C-terminal regulatory domain in an inward-facing conformation

6Z3Z の概要

• エントリーDOI: 10.2210/pdb6z3z/pdb
• EMDBエントリー: 11067
• 分子名称: Sodium/hydrogen exchanger (1 entity in total)
• 機能のキーワード: transport protein, membrane protein
• 由来する生物種: Equus caballus (Horse)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 105144.45

構造登録者

Winkelmann, I.,Matsuoka, R.,Meier, P.,Drew, D. (登録日: 2020-05-22, 公開日: 2020-11-04, 最終更新日: 2025-07-02)

主引用文献

Winklemann, I.,Matsuoka, R.,Meier, P.F.,Shutin, D.,Zhang, C.,Orellana, L.,Sexton, R.,Landreh, M.,Robinson, C.V.,Beckstein, O.,Drew, D.
Structure and elevator mechanism of the mammalian sodium/proton exchanger NHE9.
Embo J., 39:e105908-e105908, 2020

PubMed Abstract: Na /H exchangers (NHEs) are ancient membrane-bound nanomachines that work to regulate intracellular pH, sodium levels and cell volume. NHE activities contribute to the control of the cell cycle, cell proliferation, cell migration and vesicle trafficking. NHE dysfunction has been linked to many diseases, and they are targets of pharmaceutical drugs. Despite their fundamental importance to cell homeostasis and human physiology, structural information for the mammalian NHE was lacking. Here, we report the cryogenic electron microscopy structure of NHE isoform 9 (SLC9A9) from Equus caballus at 3.2 Å resolution, an endosomal isoform highly expressed in the brain and associated with autism spectrum (ASD) and attention deficit hyperactivity (ADHD) disorders. Despite low sequence identity, the NHE9 architecture and ion-binding site are remarkably similar to distantly related bacterial Na /H  antiporters with 13 transmembrane segments. Collectively, we reveal the conserved architecture of the NHE ion-binding site, their elevator-like structural transitions, the functional implications of autism disease mutations and the role of phosphoinositide lipids to promote homodimerization that, together, have important physiological ramifications.

PubMed: 33118634
DOI: 10.15252/embj.2020105908

実験手法

ELECTRON MICROSCOPY (3.19 Å)