6Z3K

Structure of protective antibody 38-1-10A Fab

6Z3K の概要

• エントリーDOI: 10.2210/pdb6z3k/pdb
• 関連するPDBエントリー: 6Z3P 6Z3Q
• 分子名称: Heavy chain, Light Chain, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: ev71, antibody 38-3-11a, antibody 38-1-10a, ev71-fab complex, immune system
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 18
• 化学式量合計: 440939.50

構造登録者

Zhou, D.,Fry, E.E.,Ren, J.,Stuart, D.I. (登録日: 2020-05-20, 公開日: 2020-09-02, 最終更新日: 2024-11-06)

主引用文献

Huang, K.A.,Zhou, D.,Fry, E.E.,Kotecha, A.,Huang, P.N.,Yang, S.L.,Tsao, K.C.,Huang, Y.C.,Lin, T.Y.,Ren, J.,Stuart, D.I.
Structural and functional analysis of protective antibodies targeting the threefold plateau of enterovirus 71.
Nat Commun, 11:5253-5253, 2020

PubMed Abstract: Enterovirus 71 (EV71)-neutralizing antibodies correlate with protection and have potential as therapeutic agents. We isolate and characterize a panel of plasmablast-derived monoclonal antibodies from an infected child whose antibody response focuses on the plateau epitope near the icosahedral 3-fold axes. Eight of a total of 19 antibodies target this epitope and three of these potently neutralize the virus. Representative neutralizing antibodies 38-1-10A and 38-3-11A both confer effective protection against lethal EV71 challenge in hSCARB2-transgenic mice. The cryo-electron microscopy structures of the EV71 virion in complex with Fab fragments of these potent and protective antibodies reveal the details of a conserved epitope formed by residues in the BC and HI loops of VP2 and the BC and HI loops of VP3 spanning the region around the 3-fold axis. Remarkably, the two antibodies interact with the epitope in quite distinct ways. These plateau-binding antibodies provide templates for promising candidate therapeutics.

PubMed: 33067459
DOI: 10.1038/s41467-020-19013-3

実験手法

X-RAY DIFFRACTION (2.7 Å)