6YYM

Structure of S. pombe Mei2 RRM3 domain bound to RNA

6YYM の概要

• エントリーDOI: 10.2210/pdb6yym/pdb
• 分子名称: Meiosis protein mei2, RNA (5'-R(*GP*CP*UP*UP*UP*UP*UP*GP*UP*UP*CP*G)-3') (3 entities in total)
• 機能のキーワード: meiosis rrm domain gene expression rna-binding domain, rna binding protein
• 由来する生物種: Schizosaccharomyces pombe 972h-; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 24346.63

構造登録者

Hazra, D.,Graille, M. (登録日: 2020-05-05, 公開日: 2020-12-23, 最終更新日: 2024-01-24)

主引用文献

Andric, V.,Nevers, A.,Hazra, D.,Auxilien, S.,Menant, A.,Graille, M.,Palancade, B.,Rougemaille, M.
A scaffold lncRNA shapes the mitosis to meiosis switch.
Nat Commun, 12:770-770, 2021

PubMed Abstract: Long non-coding RNAs (lncRNAs) contribute to the regulation of gene expression in response to intra- or extracellular signals but the underlying molecular mechanisms remain largely unexplored. Here, we identify an uncharacterized lncRNA as a central player in shaping the meiotic gene expression program in fission yeast. We report that this regulatory RNA, termed mamRNA, scaffolds the antagonistic RNA-binding proteins Mmi1 and Mei2 to ensure their reciprocal inhibition and fine tune meiotic mRNA degradation during mitotic growth. Mechanistically, mamRNA allows Mmi1 to target Mei2 for ubiquitin-mediated downregulation, and conversely enables accumulating Mei2 to impede Mmi1 activity, thereby reinforcing the mitosis to meiosis switch. These regulations also occur within a unique Mmi1-containing nuclear body, positioning mamRNA as a spatially-confined sensor of Mei2 levels. Our results thus provide a mechanistic basis for the mutual control of gametogenesis effectors and further expand our vision of the regulatory potential of lncRNAs.

PubMed: 33536434
DOI: 10.1038/s41467-021-21032-7

実験手法

X-RAY DIFFRACTION (2.63 Å)