6YXD

Room temperature structure of human adiponectin receptor 2 (ADIPOR2) at 2.9 A resolution determined by Serial Crystallography (SSX) using CrystalDirect

6YXD の概要

• エントリーDOI: 10.2210/pdb6yxd/pdb
• 関連するPDBエントリー: 5LWY
• 分子名称: Adiponectin receptor protein 2, V REGION HEAVY CHAIN, V REGION LIGHT CHAIN, ... (8 entities in total)
• 機能のキーワード: adiponectin receptor, adipor, serial synchrotron crystallography, ssx, crystaldirect, lcp crystallization, in meso, membrane proteins, membrane protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 60128.94

構造登録者

Healey, R.D.,Basu, S.,Humm, A.S.,Leyrat, C.,Dupeux, F.,Pica, A.,Granier, S.,Marquez, J.A. (登録日: 2020-05-01, 公開日: 2021-05-12, 最終更新日: 2024-11-20)

主引用文献

Healey, R.D.,Basu, S.,Humm, A.S.,Leyrat, C.,Cong, X.,Golebiowski, J.,Dupeux, F.,Pica, A.,Granier, S.,Marquez, J.A.
An automated platform for structural analysis of membrane proteins through serial crystallography.
Cell Rep Methods, 1:None-None, 2021

PubMed Abstract: Membrane proteins are central to many pathophysiological processes, yet remain very difficult to analyze structurally. Moreover, high-throughput structure-based drug discovery has not yet been exploited for membrane proteins because of lack of automation. Here, we present a facile and versatile platform for membrane protein crystallization, enabling rapid atomic structure determination at both cryogenic and room temperatures. We apply this approach to human integral membrane proteins, which allowed us to identify different conformational states of intramembrane enzyme-product complexes and analyze by molecular dynamics simulations the structural dynamics of the ADIPOR2 integral membrane protein. Finally, we demonstrate an automated pipeline combining high-throughput microcrystal soaking, automated laser-based harvesting, and serial crystallography, enabling screening of small-molecule libraries with membrane protein crystals grown . This approach brings needed automation to this important class of drug targets and enables high-throughput structure-based ligand discovery with membrane proteins.

PubMed: 34723237
DOI: 10.1016/j.crmeth.2021.100102

実験手法

X-RAY DIFFRACTION (2.9 Å)