6YVG

Crystal structure of MesI (Lpg2505) from Legionella pneumophila

6YVG の概要

• エントリーDOI: 10.2210/pdb6yvg/pdb
• 分子名称: MesI (Lpg2505), 1,2-ETHANEDIOL, IODIDE ION, ... (4 entities in total)
• 機能のキーワード: protein binding
• 由来する生物種: Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35352.45

構造登録者

Machtens, D.A.,Willerding, J.M.,Eschenburg, S.,Reubold, T.F. (登録日: 2020-04-28, 公開日: 2020-06-10, 最終更新日: 2024-05-15)

主引用文献

Machtens, D.A.,Willerding, J.M.,Eschenburg, S.,Reubold, T.F.
Crystal structure of the metaeffector MesI (Lpg2505) from Legionella pneumophila.
Biochem.Biophys.Res.Commun., 527:696-701, 2020

PubMed Abstract: Persistence and replication of the gram-negative bacterium Legionella pneumophila in the human host cell depend on so-called effector proteins that target diverse cellular functions and modulate them in favor of the pathogen. We solved the crystal structure of the L. pneumophila effector protein MesI de novo to a resolution of 2.2 Å. The 34 kDa polypeptide chain folds into two distinct α-helical domains. The larger C-terminal domain shows similarity to tetratricopeptide repeat proteins. Using size-exclusion chromatography, we confirmed that MesI binds tightly to full-length SidI and that deletion of either the N- or the C-terminus weakens the interaction. Based on the three-dimensional structure of MesI we suggest a possible binding mode for SidI and identified two homologs of MesI within the proteome of L. pneumophila that do not bind to SidI, but may act as specific inhibitors of other yet to be identified effectors.

PubMed: 32423822
DOI: 10.1016/j.bbrc.2020.05.027

実験手法

X-RAY DIFFRACTION (2.2 Å)