6YSA

Crystal structure of Arabidopsis thaliana legumain isoform beta in zymogen state

6YSA の概要

• エントリーDOI: 10.2210/pdb6ysa/pdb
• 分子名称: Vacuolar-processing enzyme beta-isozyme, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: protease, ligase, proenzyme, vacuolar processing enzyme, vpe, hydrolase
• 由来する生物種: Arabidopsis thaliana (thale cress)
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 618546.61

構造登録者

Dall, E.,Zauner, F.B.,Brandstetter, H. (登録日: 2020-04-21, 公開日: 2020-07-29, 最終更新日: 2024-11-13)

主引用文献

Dall, E.,Zauner, F.B.,Soh, W.T.,Demir, F.,Dahms, S.O.,Cabrele, C.,Huesgen, P.F.,Brandstetter, H.
Structural and functional studies ofArabidopsis thalianalegumain beta reveal isoform specific mechanisms of activation and substrate recognition.
J.Biol.Chem., 295:13047-13064, 2020

PubMed Abstract: The vacuolar cysteine protease legumain plays important functions in seed maturation and plant programmed cell death. Because of their dual protease and ligase activity, plant legumains have become of particular biotechnological interest, for the synthesis of cyclic peptides for drug design or for protein engineering. However, the molecular mechanisms behind their dual protease and ligase activities are still poorly understood, limiting their applications. Here, we present the crystal structure of legumain isoform β (AtLEGβ) in its zymogen state. Combining structural and biochemical experiments, we show for the first time that plant legumains encode distinct, isoform-specific activation mechanisms. Whereas the autocatalytic activation of isoform γ (AtLEGγ) is controlled by the latency-conferring dimer state, the activation of the monomeric AtLEGβ is concentration independent. Additionally, in AtLEGβ the plant-characteristic two-chain intermediate state is stabilized by hydrophobic rather than ionic interactions, as in AtLEGγ, resulting in significantly different pH stability profiles. The crystal structure of AtLEGβ revealed unrestricted nonprime substrate binding pockets, consistent with the broad substrate specificity, as determined by degradomic assays. Further to its protease activity, we show that AtLEGβ exhibits a true peptide ligase activity. Whereas cleavage-dependent transpeptidase activity has been reported for other plant legumains, AtLEGβ is the first example of a plant legumain capable of linking free termini. The discovery of these isoform-specific differences will allow us to identify and rationally design efficient ligases with application in biotechnology and drug development.

PubMed: 32719006
DOI: 10.1074/jbc.RA120.014478

実験手法

X-RAY DIFFRACTION (2.01 Å)