6YQF

Crystal structure of the SYCE2-TEX12 delta-Ctip complex in a 4:4 assembly

6YQF の概要

• エントリーDOI: 10.2210/pdb6yqf/pdb
• 関連するPDBエントリー: 6R17
• 分子名称: Synaptonemal complex central element protein 2, Testis-expressed protein 12 (2 entities in total)
• 機能のキーワード: synaptonemal complex meiosis recombination coiled-coil self-assembly syce2 tex12 structural protein, structural protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 42255.97

構造登録者

Dunce, J.M.,Davies, O.R. (登録日: 2020-04-16, 公開日: 2021-04-28, 最終更新日: 2024-01-24)

主引用文献

Dunce, J.M.,Salmon, L.J.,Davies, O.R.
Structural basis of meiotic chromosome synaptic elongation through hierarchical fibrous assembly of SYCE2-TEX12.
Nat.Struct.Mol.Biol., 28:681-693, 2021

PubMed Abstract: The synaptonemal complex (SC) is a supramolecular protein assembly that mediates synapsis between homologous chromosomes during meiosis. SC elongation along the chromosome length (up to 24 μm) depends on its midline α-fibrous component SYCE2-TEX12. Here, we report X-ray crystal structures of human SYCE2-TEX12 as an individual building block and on assembly within a fibrous lattice. We combine these structures with mutagenesis, biophysics and electron microscopy to reveal the hierarchical mechanism of SYCE2-TEX12 fiber assembly. SYCE2-TEX12's building blocks are 2:2 coiled coils that dimerize into 4:4 hetero-oligomers and interact end-to-end and laterally to form 10-nm fibers that intertwine within 40-nm bundled micrometer-long fibers that define the SC's midline structure. This assembly mechanism bears striking resemblance with intermediate filament proteins vimentin, lamin and keratin. Thus, SYCE2-TEX12 exhibits behavior typical of cytoskeletal proteins to provide an α-fibrous SC backbone that structurally underpins synaptic elongation along meiotic chromosomes.

PubMed: 34373646
DOI: 10.1038/s41594-021-00636-z

実験手法

X-RAY DIFFRACTION (3.33 Å)