6YP9

Rabbit muscle actin in complex with ADF-H and ATP-ATTO-488

6YP9 の概要

• エントリーDOI: 10.2210/pdb6yp9/pdb
• 分子名称: Actin, alpha skeletal muscle, Twinfilin-1, ADENOSINE-5'-TRIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: actin, adf-h domain, atp-atto-488, protein binding
• 由来する生物種: Mus musculus (House mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 58862.64

構造登録者

Kogan, K.,Kotila, T.,Lappalainen, P. (登録日: 2020-04-15, 公開日: 2020-12-09, 最終更新日: 2024-01-24)

主引用文献

Colombo, J.,Antkowiak, A.,Kogan, K.,Kotila, T.,Elliott, J.,Guillotin, A.,Lappalainen, P.,Michelot, A.
A functional family of fluorescent nucleotide analogues to investigate actin dynamics and energetics.
Nat Commun, 12:548-548, 2021

PubMed Abstract: Actin polymerization provides force for vital processes of the eukaryotic cell, but our understanding of actin dynamics and energetics remains limited due to the lack of high-quality probes. Most current probes affect dynamics of actin or its interactions with actin-binding proteins (ABPs), and cannot track the bound nucleotide. Here, we identify a family of highly sensitive fluorescent nucleotide analogues structurally compatible with actin. We demonstrate that these fluorescent nucleotides bind to actin, maintain functional interactions with a number of essential ABPs, are hydrolyzed within actin filaments, and provide energy to power actin-based processes. These probes also enable monitoring actin assembly and nucleotide exchange with single-molecule microscopy and fluorescence anisotropy kinetics, therefore providing robust and highly versatile tools to study actin dynamics and functions of ABPs.

PubMed: 33483497
DOI: 10.1038/s41467-020-20827-4

実験手法

X-RAY DIFFRACTION (2.564 Å)