6YMX

CIII2/CIV respiratory supercomplex from Saccharomyces cerevisiae

6YMX の概要

• エントリーDOI: 10.2210/pdb6ymx/pdb
• EMDBエントリー: 10847
• 分子名称: Cytochrome c oxidase subunit 1, Cytochrome c oxidase subunit 12, mitochondrial, Cytochrome c oxidase subunit 13, mitochondrial, ... (40 entities in total)
• 機能のキーワード: complex, ciii, civ, supercomplex, electron transport
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c); 詳細
• タンパク質・核酸の鎖数: 32
• 化学式量合計: 669786.90

構造登録者

Berndtsson, J.,Rathore, S.,Ott, M. (登録日: 2020-04-10, 公開日: 2020-09-09, 最終更新日: 2021-03-24)

主引用文献

Berndtsson, J.,Aufschnaiter, A.,Rathore, S.,Marin-Buera, L.,Dawitz, H.,Diessl, J.,Kohler, V.,Barrientos, A.,Buttner, S.,Fontanesi, F.,Ott, M.
Respiratory supercomplexes enhance electron transport by decreasing cytochrome c diffusion distance.
Embo Rep., 21:e51015-e51015, 2020

PubMed Abstract: Respiratory chains are crucial for cellular energy conversion and consist of multi-subunit complexes that can assemble into supercomplexes. These structures have been intensively characterized in various organisms, but their physiological roles remain unclear. Here, we elucidate their function by leveraging a high-resolution structural model of yeast respiratory supercomplexes that allowed us to inhibit supercomplex formation by mutation of key residues in the interaction interface. Analyses of a mutant defective in supercomplex formation, which still contains fully functional individual complexes, show that the lack of supercomplex assembly delays the diffusion of cytochrome c between the separated complexes, thus reducing electron transfer efficiency. Consequently, competitive cellular fitness is severely reduced in the absence of supercomplex formation and can be restored by overexpression of cytochrome c. In sum, our results establish how respiratory supercomplexes increase the efficiency of cellular energy conversion, thereby providing an evolutionary advantage for aerobic organisms.

PubMed: 33016568
DOI: 10.15252/embr.202051015

実験手法

ELECTRON MICROSCOPY (3.17 Å)