6YMC

26-mer stem-loop RNA

6YMC の概要

• エントリーDOI: 10.2210/pdb6ymc/pdb
• 分子名称: RNA (26-MER), BARIUM ION (3 entities in total)
• 機能のキーワード: rna, tetra-loop
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 18111.31

構造登録者

Janowski, R.,Niessing, D. (登録日: 2020-04-08, 公開日: 2022-04-20, 最終更新日: 2025-03-12)

主引用文献

Niedner-Boblenz, A.,Monecke, T.,Hennig, J.,Klostermann, M.,Hofweber, M.,Davydova, E.,Gerber, A.P.,Anosova, I.,Mayer, W.,Muller, M.,Heym, R.G.,Janowski, R.,Paillart, J.C.,Dormann, D.,Zarnack, K.,Sattler, M.,Niessing, D.
Intrinsically disordered RNA-binding motifs cooperate to catalyze RNA folding and drive phase separation.
Nucleic Acids Res., 52:14205-14228, 2024

PubMed Abstract: RNA-binding proteins are essential for gene regulation and the spatial organization of cells. Here, we report that the yeast ribosome biogenesis factor Loc1p is an intrinsically disordered RNA-binding protein with eight repeating positively charged, unstructured nucleic acid binding (PUN) motifs. While a single of these previously undefined motifs stabilizes folded RNAs, multiple copies strongly cooperate to catalyze RNA folding. In the presence of RNA, these multivalent PUN motifs drive phase separation. Proteome-wide searches in pro- and eukaryotes for proteins with similar arrays of PUN motifs reveal a strong enrichment in RNA-mediated processes and DNA remodeling. Thus, PUN motifs are potentially involved in a large variety of RNA- and DNA-related processes by concentrating them in membraneless organelles. The general function and wide distribution of PUN motifs across species suggest that in an ancient 'RNA world' PUN-like motifs may have supported the correct folding of early ribozymes.

PubMed: 39558160
DOI: 10.1093/nar/gkae1107

実験手法

X-RAY DIFFRACTION (2 Å)