6YLP

EGFP_in_Acidic_env Directionality of Optical Properties of Fluorescent Proteins

6YLP の概要

• エントリーDOI: 10.2210/pdb6ylp/pdb
• 分子名称: eGFP_in_Acidic_env, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: fluorescent protein, egfp
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28650.13

構造登録者

Myskova, J.,Rybakova, O.,Brynda, J.,Lazar, J. (登録日: 2020-04-07, 公開日: 2020-12-16, 最終更新日: 2024-10-23)

主引用文献

Myskova, J.,Rybakova, O.,Brynda, J.,Khoroshyy, P.,Bondar, A.,Lazar, J.
Directionality of light absorption and emission in representative fluorescent proteins.
Proc.Natl.Acad.Sci.USA, 117:32395-32401, 2020

PubMed Abstract: Fluorescent molecules are like antennas: The rate at which they absorb light depends on their orientation with respect to the incoming light wave, and the apparent intensity of their emission depends on their orientation with respect to the observer. However, the directions along which the most important fluorescent molecules in biology, fluorescent proteins (FPs), absorb and emit light are generally not known. Our optical and X-ray investigations of FP crystals have now allowed us to determine the molecular orientations of the excitation and emission transition dipole moments in the FPs mTurquoise2, eGFP, and mCherry, and the photoconvertible FP mEos4b. Our results will allow using FP directionality in studies of molecular and biological processes, but also in development of novel bioengineering and bioelectronics applications.

PubMed: 33273123
DOI: 10.1073/pnas.2017379117

実験手法

X-RAY DIFFRACTION (1.55 Å)