6YJJ

Structure of CYRI-B (FAM49B) from Rhincodon typus (selenomethionine derivative)

6YJJ の概要

• エントリーDOI: 10.2210/pdb6yjj/pdb
• 分子名称: CYRI-B (FAM49B) (2 entities in total)
• 機能のキーワード: cyri-b, fam49b, actin assembly, rac binding protein, cell motility regulator, cytosolic protein
• 由来する生物種: Rhincodon typus (Whale shark)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37378.85

構造登録者

Kaplan, E. (登録日: 2020-04-03, 公開日: 2020-10-14, 最終更新日: 2024-10-23)

主引用文献

Kaplan, E.,Stone, R.,Hume, P.J.,Greene, N.P.,Koronakis, V.
Structure of CYRI-B (FAM49B), a key regulator of cellular actin assembly.
Acta Crystallogr D Struct Biol, 76:1015-1024, 2020

PubMed Abstract: In eukaryotes, numerous fundamental processes are controlled by the WAVE regulatory complex (WRC) that regulates cellular actin polymerization, crucial for cell motility, cell-cell adhesion and epithelial differentiation. Actin assembly is triggered by interaction of the small GTPase Rac1 with CYFIP1, a key component of the WRC. Previously known as FAM49B, CYRI-B is a protein that is highly conserved across the Eukaryota and has recently been revealed to be a key regulator of Rac1 activity. Mutation of CYRI-B or alteration of its expression therefore leads to altered actin nucleation dynamics, with impacts on lamellipodia formation, cell migration and infection by intracellular pathogens. In addition, knockdown of CYRI-B expression in cancer cell lines results in accelerated cell proliferation and invasiveness. Here, the structure of Rhincodon typus (whale shark) CYRI-B is presented, which is the first to be reported of any CYRI family member. Solved by X-ray crystallography, the structure reveals that CYRI-B comprises three distinct α-helical subdomains and is highly structurally related to a conserved domain present in CYFIP proteins. The work presented here establishes a template towards a better understanding of CYRI-B biological function.

PubMed: 33021503
DOI: 10.1107/S2059798320010906

実験手法

X-RAY DIFFRACTION (2.4 Å)