6YHU

Crystal structure of the nsp7-nsp8 complex of SARS-CoV-2

6YHU の概要

• エントリーDOI: 10.2210/pdb6yhu/pdb
• 分子名称: Replicase polyprotein 1a (3 entities in total)
• 機能のキーワード: nsp7, nsp8, sars-cov-2, ncov-2019, covid-19, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 41636.16

構造登録者

Konkolova, E.,Klima, M.,Boura, E. (登録日: 2020-03-31, 公開日: 2020-04-29, 最終更新日: 2024-11-06)

主引用文献

Konkolova, E.,Klima, M.,Nencka, R.,Boura, E.
Structural analysis of the putative SARS-CoV-2 primase complex.
J.Struct.Biol., 211:107548-107548, 2020

PubMed Abstract: We report the crystal structure of the SARS-CoV-2 putative primase composed of the nsp7 and nsp8 proteins. We observed a dimer of dimers (2:2 nsp7-nsp8) in the crystallographic asymmetric unit. The structure revealed a fold with a helical core of the heterotetramer formed by both nsp7 and nsp8 that is flanked with two symmetry-related nsp8 β-sheet subdomains. It was also revealed that two hydrophobic interfaces one of approx. 1340 Å connects the nsp7 to nsp8 and a second one of approx. 950 Å connects the dimers and form the observed heterotetramer. Interestingly, analysis of the surface electrostatic potential revealed a putative RNA binding site that is formed only within the heterotetramer.

PubMed: 32535228
DOI: 10.1016/j.jsb.2020.107548

実験手法

X-RAY DIFFRACTION (2 Å)