6YFQ

Virus-like particle of bacteriophage NT-214

これはPDB形式変換不可エントリーです。

6YFQ の概要

• エントリーDOI: 10.2210/pdb6yfq/pdb
• 分子名称: coat protein (1 entity in total)
• 機能のキーワード: virus, virus-like particle, structural protein, virus like particle
• 由来する生物種: Leviviridae sp.
• タンパク質・核酸の鎖数: 60
• 化学式量合計: 871471.20

構造登録者

Rumnieks, J.,Kalnins, G.,Sisovs, M.,Lieknina, I.,Tars, K. (登録日: 2020-03-26, 公開日: 2020-09-02, 最終更新日: 2024-10-23)

主引用文献

Rumnieks, J.,Lieknina, I.,Kalnins, G.,Sisovs, M.,Akopjana, I.,Bogans, J.,Tars, K.
Three-dimensional structure of 22 uncultured ssRNA bacteriophages: Flexibility of the coat protein fold and variations in particle shapes.
Sci Adv, 6:-, 2020

PubMed Abstract: The single-stranded RNA (ssRNA) bacteriophages are among the simplest known viruses with small genomes and exceptionally high mutation rates. The number of ssRNA phage isolates has remained very low, but recent metagenomic studies have uncovered an immense variety of distinct uncultured ssRNA phages. The coat proteins (CPs) in these genomes are particularly diverse, with notable variation in length and often no recognizable similarity to previously known viruses. We recombinantly expressed metagenome-derived ssRNA phage CPs to produce virus-like particles and determined the three-dimensional structure of 22 previously uncharacterized ssRNA phage capsids covering nine distinct CP types. The structures revealed substantial deviations from the previously known ssRNA phage CP fold, uncovered an unusual prolate particle shape, and revealed a previously unseen dsRNA binding mode. These data expand our knowledge of the evolution of viral structural proteins and are of relevance for applications such as ssRNA phage-based vaccine design.

PubMed: 32917600
DOI: 10.1126/sciadv.abc0023

実験手法

X-RAY DIFFRACTION (3.8 Å)