6YE8

E.coli's Putrescine receptor PotF complexed with Spermidine

6YE8 の概要

• エントリーDOI: 10.2210/pdb6ye8/pdb
• 関連するPDBエントリー: 6YE0 6YE6 6YE7
• 分子名称: Putrescine-binding periplasmic protein, SPERMIDINE, (2R)-1-methoxypropan-2-amine, ... (7 entities in total)
• 機能のキーワード: periplasmic binding protein, e.coli, spermidine, transport protein
• 由来する生物種: Escherichia coli K-12
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 80088.85

構造登録者

Shanmugaratnam, S.,Kroeger, P.,Hocker, B. (登録日: 2020-03-24, 公開日: 2021-01-20, 最終更新日: 2024-11-06)

主引用文献

Kroger, P.,Shanmugaratnam, S.,Ferruz, N.,Schweimer, K.,Hocker, B.
A comprehensive binding study illustrates ligand recognition in the periplasmic binding protein PotF.
Structure, 29:433-, 2021

PubMed Abstract: Periplasmic binding proteins (PBPs) are ubiquitous receptors in gram-negative bacteria. They sense solutes and play key roles in nutrient uptake. Escherichia coli's putrescine receptor PotF has been reported to bind putrescine and spermidine. We reveal that several similar biogenic polyamines are recognized by PotF. Using isothermal titration calorimetry paired with X-ray crystallography of the different complexes, we unveil PotF's binding modes in detail. The binding site for PBPs is located between two lobes that undergo a large conformational change upon ligand recognition. Hence, analyzing the influence of ligands on complex formation is crucial. Therefore, we solved crystal structures of an open and closed apo state and used them as a basis for molecular dynamics simulations. In addition, we accessed structural behavior in solution for all complexes by H-N HSQC NMR spectroscopy. This combined analysis provides a robust framework for understanding ligand binding for future developments in drug design and protein engineering.

PubMed: 33406388
DOI: 10.1016/j.str.2020.12.005

実験手法

X-RAY DIFFRACTION (1.5 Å)