6YBV

Structure of a human 48S translational initiation complex - eIF2-TC

6YBV の概要

• エントリーDOI: 10.2210/pdb6ybv/pdb
• EMDBエントリー: 10774
• 分子名称: Eukaryotic translation initiation factor 2 subunit 2, Eukaryotic translation initiation factor 2 subunit 1, Initiator methionine tRNA, ... (5 entities in total)
• 機能のキーワード: ribosome, translation, initiation complex
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 150968.24

構造登録者

Brito Querido, J.,Sokabe, M.,Kraatz, S.,Gordiyenko, Y.,Skehel, M.,Fraser, C.,Ramakrishnan, V. (登録日: 2020-03-17, 公開日: 2020-09-16, 最終更新日: 2024-05-22)

主引用文献

Brito Querido, J.,Sokabe, M.,Kraatz, S.,Gordiyenko, Y.,Skehel, J.M.,Fraser, C.S.,Ramakrishnan, V.
Structure of a human 48Stranslational initiation complex.
Science, 369:1220-1227, 2020

PubMed Abstract: A key step in translational initiation is the recruitment of the 43 preinitiation complex by the cap-binding complex [eukaryotic initiation factor 4F (eIF4F)] at the 5' end of messenger RNA (mRNA) to form the 48 initiation complex (i.e., the 48). The 48 then scans along the mRNA to locate a start codon. To understand the mechanisms involved, we used cryo-electron microscopy to determine the structure of a reconstituted human 48 The structure reveals insights into early events of translation initiation complex assembly, as well as how eIF4F interacts with subunits of eIF3 near the mRNA exit channel in the 43 The location of eIF4F is consistent with a slotting model of mRNA recruitment and suggests that downstream mRNA is unwound at least in part by being "pulled" through the 40 subunit during scanning.

PubMed: 32883864
DOI: 10.1126/science.aba4904

実験手法

ELECTRON MICROSCOPY (3.8 Å)