6Y76

AP01 - a redesigned transferrin receptor apical domain

6Y76 の概要

• エントリーDOI: 10.2210/pdb6y76/pdb
• 分子名称: Transferrin receptor protein 1, SODIUM ION (3 entities in total)
• 機能のキーワード: transferrin receptor, transferrin, protein design, rosetta, cytosolic protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36173.96

構造登録者

Oberdorfer, G.,Berger, S.A.,Bjelic, S.,Sjostrom, D.J. (登録日: 2020-02-28, 公開日: 2020-07-22, 最終更新日: 2024-10-09)

主引用文献

Sjostrom, D.J.,Berger, S.A.,Oberdorfer, G.,Bjelic, S.
Computational backbone design enables soluble engineering of transferrin receptor apical domain.
Proteins, 88:1569-1577, 2020

PubMed Abstract: Supply of iron into human cells is achieved by iron carrier protein transferrin and its receptor that upon complex formation get internalized by endocytosis. Similarly, the iron needs to be delivered into the brain, and necessitates the transport across the blood-brain barrier. While there are still unanswered questions about these mechanisms, extensive efforts have been made to use the system for delivery of therapeutics into biological compartments. The dimeric form of the receptor, where each subunit consists of three domains, further complicates the detailed investigation of molecular determinants responsible for guiding the receptor interactions with other proteins. Especially the apical domain's biological function has been elusive. To further the study of transferrin receptor, we have computationally decoupled the apical domain for soluble expression, and validated the design strategy by structure determination. Besides presenting a methodology for solubilizing domains, the results will allow for study of apical domain's function.

PubMed: 32592192
DOI: 10.1002/prot.25974

実験手法

X-RAY DIFFRACTION (1.98 Å)