6Y57

Structure of human ribosome in hybrid-PRE state

これはPDB形式変換不可エントリーです。

6Y57 の概要

• エントリーDOI: 10.2210/pdb6y57/pdb
• 関連するPDBエントリー: 6Y0G 6Y2L
• EMDBエントリー: 10668 10674 10690
• 分子名称: mRNA, 60S ribosomal protein L5, 60S ribosomal protein L6, ... (85 entities in total)
• 機能のキーワード: hybrid-pre state, ribosome
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 82
• 化学式量合計: 3837443.72

構造登録者

Bhaskar, V.,Schenk, A.D.,Cavadini, S.,von Loeffelholz, O.,Natchiar, S.K.,Klaholz, B.P.,Chao, J.A. (登録日: 2020-02-25, 公開日: 2020-04-15, 最終更新日: 2024-05-22)

主引用文献

Bhaskar, V.,Graff-Meyer, A.,Schenk, A.D.,Cavadini, S.,von Loeffelholz, O.,Natchiar, S.K.,Artus-Revel, C.G.,Hotz, H.R.,Bretones, G.,Klaholz, B.P.,Chao, J.A.
Dynamics of uS19 C-Terminal Tail during the Translation Elongation Cycle in Human Ribosomes.
Cell Rep, 31:107473-107473, 2020

PubMed Abstract: Ribosomes undergo multiple conformational transitions during translation elongation. Here, we report the high-resolution cryoelectron microscopy (cryo-EM) structure of the human 80S ribosome in the post-decoding pre-translocation state (classical-PRE) at 3.3-Å resolution along with the rotated (hybrid-PRE) and the post-translocation states (POST). The classical-PRE state ribosome structure reveals a previously unobserved interaction between the C-terminal region of the conserved ribosomal protein uS19 and the A- and P-site tRNAs and the mRNA in the decoding site. In addition to changes in the inter-subunit bridges, analysis of different ribosomal conformations reveals the dynamic nature of this domain and suggests a role in tRNA accommodation and translocation during elongation. Furthermore, we show that disease-associated mutations in uS19 result in increased frameshifting. Together, this structure-function analysis provides mechanistic insights into the role of the uS19 C-terminal tail in the context of mammalian ribosomes.

PubMed: 32268098
DOI: 10.1016/j.celrep.2020.03.037

実験手法

ELECTRON MICROSCOPY (3.5 Å)