6Y2N

Crystal structure of ribonucleotide reductase R2 subunit solved by serial synchrotron crystallography

Summary for 6Y2N

• Entry DOI: 10.2210/pdb6y2n/pdb
• Descriptor: Ribonucleoside-diphosphate reductase subunit beta, MANGANESE (III) ION, FE (III) ION, ... (4 entities in total)
• Functional Keywords: ribonucleotide reductase r2 subunit, mn/fe cofactor, metalloprotein oxidoreductase, ferritin-like superfamily, oxidoreductase
• Biological source: Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
• Total number of polymer chains: 1
• Total formula weight: 39719.67

Authors

Shilova, A.,Lebrette, H.,Aurelius, O.,Hogbom, M.,Mueller, U. (deposition date: 2020-02-17, release date: 2020-10-07, Last modification date: 2024-05-01)

Primary citation

Shilova, A.,Lebrette, H.,Aurelius, O.,Nan, J.,Welin, M.,Kovacic, R.,Ghosh, S.,Safari, C.,Friel, R.J.,Milas, M.,Matej, Z.,Hogbom, M.,Branden, G.,Kloos, M.,Shoeman, R.L.,Doak, B.,Ursby, T.,Hakansson, M.,Logan, D.T.,Mueller, U.
Current status and future opportunities for serial crystallography at MAX IV Laboratory.
J.Synchrotron Radiat., 27:1095-1102, 2020

PubMed Abstract: Over the last decade, serial crystallography, a method to collect complete diffraction datasets from a large number of microcrystals delivered and exposed to an X-ray beam in random orientations at room temperature, has been successfully implemented at X-ray free-electron lasers and synchrotron radiation facility beamlines. This development relies on a growing variety of sample presentation methods, including different fixed target supports, injection methods using gas-dynamic virtual-nozzle injectors and high-viscosity extrusion injectors, and acoustic levitation of droplets, each with unique requirements. In comparison with X-ray free-electron lasers, increased beam time availability makes synchrotron facilities very attractive to perform serial synchrotron X-ray crystallography (SSX) experiments. Within this work, the possibilities to perform SSX at BioMAX, the first macromolecular crystallography beamline at  MAX IV Laboratory in Lund, Sweden, are described, together with case studies from the SSX user program: an implementation of a high-viscosity extrusion injector to perform room temperature serial crystallography at BioMAX using two solid supports - silicon nitride membranes (Silson, UK) and XtalTool (Jena Bioscience, Germany). Future perspectives for the dedicated serial crystallography beamline MicroMAX at MAX IV Laboratory, which will provide parallel and intense micrometre-sized X-ray beams, are discussed.

PubMed: 32876583
DOI: 10.1107/S1600577520008735

Experimental method

X-RAY DIFFRACTION (2.4 Å)