6Y1V

Mycobacterium tuberculosis FtsZ-GTP-gamma-S in complex with 4-hydroxycoumarin

6Y1V の概要

• エントリーDOI: 10.2210/pdb6y1v/pdb
• 分子名称: Cell division protein FtsZ, DIMETHYL SULFOXIDE, 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: cell division protein, antibiotic
• 由来する生物種: Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 65805.52

構造登録者

Alnami, A.T.,Norton, R.S.,Pena, H.P.,Haider, M.,kozielski, F. (登録日: 2020-02-13, 公開日: 2021-06-09, 最終更新日: 2024-01-24)

主引用文献

Alnami, A.,Norton, R.S.,Pena, H.P.,Haider, S.,Kozielski, F.
Conformational Flexibility of A Highly Conserved Helix Controls Cryptic Pocket Formation in FtsZ.
J.Mol.Biol., 433:167061-167061, 2021

PubMed Abstract: Mycobacterium tuberculosis is responsible for more than 1.6 million deaths each year. One potential antibacterial target in M. tuberculosis is filamentous temperature sensitive protein Z (FtsZ), which is the bacterial homologue of mammalian tubulin, a validated cancer target. M. tuberculosis FtsZ function is essential, with its inhibition leading to arrest of cell division, elongation of the bacterial cell and eventual cell death. However, the development of potent inhibitors against FtsZ has been a challenge owing to the lack of structural information. Here we report multiple crystal structures of M. tuberculosis FtsZ in complex with a coumarin analogue. The 4-hydroxycoumarin binds exclusively to two novel cryptic pockets in nucleotide-free FtsZ, but not to the binary FtsZ-GTP or GDP complexes. Our findings provide a detailed understanding of the molecular basis for cryptic pocket formation, controlled by the conformational flexibility of the H7 helix, and thus reveal an important structural and mechanistic rationale for coumarin antibacterial activity.

PubMed: 34023403
DOI: 10.1016/j.jmb.2021.167061

実験手法

X-RAY DIFFRACTION (2.4 Å)