6Y0K

Sulfite oxidase from Thermus thermophilus with coordinated phosphate

これはPDB形式変換不可エントリーです。

6Y0K の概要

• エントリーDOI: 10.2210/pdb6y0k/pdb
• 分子名称: Putaitve sulfite oxidase, (MOLYBDOPTERIN-S,S)-OXO-MOLYBDENUM, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: molybdoenzyme, sulfite oxidase, oxidoreductase
• 由来する生物種: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 44409.56

構造登録者

Djeghader, A.,Soulimane, T. (登録日: 2020-02-08, 公開日: 2020-08-05, 最終更新日: 2024-01-24)

主引用文献

Djeghader, A.,Rossotti, M.,Abdulkarim, S.,Biaso, F.,Gerbaud, G.,Nitschke, W.,Schoepp-Cothenet, B.,Soulimane, T.,Grimaldi, S.
Structural evidence for a reaction intermediate mimic in the active site of a sulfite dehydrogenase.
Chem.Commun.(Camb.), 56:9850-9853, 2020

PubMed Abstract: By combining X-ray crystallography, electron paramagnetic resonance techniques and density functional theory-based modelling, we provide evidence for a direct coordination of the product analogue, phosphate, to the molybdenum active site of a sulfite dehydrogenase. This interaction is mimicking the still experimentally uncharacterized reaction intermediate proposed to arise during the catalytic cycle of this class of enzymes. This work opens new perspectives for further deciphering the reaction mechanism of this nearly ubiquitous class of oxidoreductases.

PubMed: 32716419
DOI: 10.1039/d0cc03634j

実験手法

X-RAY DIFFRACTION (1.7 Å)