6Y0H

High resolution structure of GH11 xylanase from Nectria haematococca

6Y0H の概要

• エントリーDOI: 10.2210/pdb6y0h/pdb
• 分子名称: Endo-1,4-beta-xylanase (2 entities in total)
• 機能のキーワード: xylanase, atomic resolution, nectria haematococca, hydrolase
• 由来する生物種: [Nectria] haematococca mpVI 77-13-4
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20917.43

構造登録者

Andaleeb, H.,Betzel, C.,Perbandt, M.,Brognaro, H. (登録日: 2020-02-07, 公開日: 2020-10-14, 最終更新日: 2024-01-24)

主引用文献

Andaleeb, H.,Ullah, N.,Falke, S.,Perbandt, M.,Brognaro, H.,Betzel, C.
High-resolution crystal structure and biochemical characterization of a GH11 endoxylanase from Nectria haematococca.
Sci Rep, 10:15658-15658, 2020

PubMed Abstract: Enzymatic degradation of vegetal biomass offers versatile procedures to improve the production of alternative fuels and other biomass-based products. Here we present the three-dimensional structure of a xylanase from Nectria haematococca (NhGH11) at 1.0 Å resolution and its functional properties. The atomic resolution structure provides details and insights about the complex hydrogen bonding network of the active site region and allowed a detailed comparison with homologous structures. Complementary biochemical studies showed that the xylanase can catalyze the hydrolysis of complex xylan into simple xylose aldopentose subunits of different lengths. NhGH11 can catalyze the efficient breakdown of beechwood xylan, xylan polysaccharide, and wheat arabinoxylan with turnover numbers of 1730.6 ± 318.1 min, 1648.2 ± 249.3 min and 2410.8 ± 517.5 min respectively. NhGH11 showed maximum catalytic activity at pH 6.0 and 45 °C. The mesophilic character of NhGH11 can be explained by distinct structural features in comparison to thermophilic GH11 enzymes, including the number of hydrogen bonds, side chain interactions and number of buried water molecules. The enzymatic activity of NhGH11 is not very sensitive to metal ions and chemical reagents that are typically present in associated industrial production processes. The data we present highlights the potential of NhGH11 to be applied in industrial biomass degradation processes.

PubMed: 32973265
DOI: 10.1038/s41598-020-72644-w

実験手法

X-RAY DIFFRACTION (1 Å)