6XYA

Cap-binding domain of SFTSV L protein

6XYA の概要

• エントリーDOI: 10.2210/pdb6xya/pdb
• 分子名称: RNA-dependent RNA polymerase, 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: bunyavirus, cap binding, cap-snatching, viral polymerase, viral protein
• 由来する生物種: SFTS virus AH12
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13405.84

構造登録者

Gogrefe, N.,Guenther, S.,Rosenthal, M. (登録日: 2020-01-30, 公開日: 2020-04-08, 最終更新日: 2024-01-24)

主引用文献

Vogel, D.,Thorkelsson, S.R.,Quemin, E.R.J.,Meier, K.,Kouba, T.,Gogrefe, N.,Busch, C.,Reindl, S.,Gunther, S.,Cusack, S.,Grunewald, K.,Rosenthal, M.
Structural and functional characterization of the severe fever with thrombocytopenia syndrome virus L protein.
Nucleic Acids Res., 48:5749-5765, 2020

PubMed Abstract: The Bunyavirales order contains several emerging viruses with high epidemic potential, including Severe fever with thrombocytopenia syndrome virus (SFTSV). The lack of medical countermeasures, such as vaccines and antivirals, is a limiting factor for the containment of any virus outbreak. To develop such antivirals a profound understanding of the viral replication process is essential. The L protein of bunyaviruses is a multi-functional and multi-domain protein performing both virus transcription and genome replication and, therefore, is an ideal drug target. We established expression and purification procedures for the full-length L protein of SFTSV. By combining single-particle electron cryo-microscopy and X-ray crystallography, we obtained 3D models covering ∼70% of the SFTSV L protein in the apo-conformation including the polymerase core region, the endonuclease and the cap-binding domain. We compared this first L structure of the Phenuiviridae family to the structures of La Crosse peribunyavirus L protein and influenza orthomyxovirus polymerase. Together with a comprehensive biochemical characterization of the distinct functions of SFTSV L protein, this work provides a solid framework for future structural and functional studies of L protein-RNA interactions and the development of antiviral strategies against this group of emerging human pathogens.

PubMed: 32313945
DOI: 10.1093/nar/gkaa253

実験手法

X-RAY DIFFRACTION (1.35 Å)