6XWX

Crystal structure of the Tof1-Csm3 complex

Summary for 6XWX

• Entry DOI: 10.2210/pdb6xwx/pdb
• Descriptor: Uncharacterized protein,Uncharacterized protein, Chromosome segregation in meiosis protein (2 entities in total)
• Functional Keywords: fork protection complex, replisome, cohesion establishment, s phase checkpoint, armadillo repeat, replication
• Biological source: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719); More
• Total number of polymer chains: 6
• Total formula weight: 247306.68

Authors

Grabarczyk, D.B. (deposition date: 2020-01-24, release date: 2020-05-27, Last modification date: 2024-01-24)

Primary citation

Grabarczyk, D.B.
Crystal structure and interactions of the Tof1-Csm3 (Timeless-Tipin) fork protection complex.
Nucleic Acids Res., 48:6996-7004, 2020

PubMed Abstract: The Tof1-Csm3 fork protection complex has a central role in the replisome-it promotes the progression of DNA replication forks and protects them when they stall, while also enabling cohesion establishment and checkpoint responses. Here, I present the crystal structure of the Tof1-Csm3 complex from Chaetomium thermophilum at 3.1 Å resolution. The structure reveals that both proteins together form an extended alpha helical repeat structure, which suggests a mechanical or scaffolding role for the complex. Expanding on this idea, I characterize a DNA interacting region and a cancer-associated Mrc1 binding site. This study provides the molecular basis for understanding the functions of the Tof1-Csm3 complex, its human orthologue the Timeless-Tipin complex and additionally the Drosophila circadian rhythm protein Timeless.

PubMed: 32469068
DOI: 10.1093/nar/gkaa456

Experimental method

X-RAY DIFFRACTION (3.1 Å)