6XUS

RNA dodecamer with a 6-hydrazino-2-aminopurine modified base

Summary for 6XUS

• Entry DOI: 10.2210/pdb6xus/pdb
• Descriptor: RNA dodecamer with a 6-hydrazino-2-aminopurine modified base, SODIUM ION, MAGNESIUM ION, ... (4 entities in total)
• Functional Keywords: 6-hydrazino-2-aminopurine modified base, rna
• Biological source: synthetic construct
• Total number of polymer chains: 1
• Total formula weight: 3951.67

Authors

Ennifar, E.,Micura, R.,Gasser, C.,Brillet, K. (deposition date: 2020-01-21, release date: 2021-02-03, Last modification date: 2024-01-24)

Primary citation

Gasser, C.,Delazer, I.,Neuner, E.,Pascher, K.,Brillet, K.,Klotz, S.,Trixl, L.,Himmelstoss, M.,Ennifar, E.,Rieder, D.,Lusser, A.,Micura, R.
Thioguanosine Conversion Enables mRNA-Lifetime Evaluation by RNA Sequencing Using Double Metabolic Labeling (TUC-seq DUAL).
Angew.Chem.Int.Ed.Engl., 59:6881-6886, 2020

PubMed Abstract: Temporal information about cellular RNA populations is essential to understand the functional roles of RNA. We have developed the hydrazine/NH Cl/OsO -based conversion of 6-thioguanosine (6sG) into A', where A' constitutes a 6-hydrazino purine derivative. A' retains the Watson-Crick base-pair mode and is efficiently decoded as adenosine in primer extension assays and in RNA sequencing. Because 6sG is applicable to metabolic labeling of freshly synthesized RNA and because the conversion chemistry is fully compatible with the conversion of the frequently used metabolic label 4-thiouridine (4sU) into C, the combination of both modified nucleosides in dual-labeling setups enables high accuracy measurements of RNA decay. This approach, termed TUC-seq DUAL, uses the two modified nucleosides in subsequent pulses and their simultaneous detection, enabling mRNA-lifetime evaluation with unprecedented precision.

PubMed: 31999864
DOI: 10.1002/anie.201916272

Experimental method

X-RAY DIFFRACTION (1.1 Å)