6XSS

CryoEM structure of designed helical fusion protein C4_nat_HFuse-7900

6XSS の概要

• エントリーDOI: 10.2210/pdb6xss/pdb
• EMDBエントリー: 22305 22306
• 分子名称: C4_nat_HFuse-7900 (1 entity in total)
• 機能のキーワード: helical bundle, helical repeat, de novo protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 114825.94

構造登録者

Redler, R.L.,Edman, N.I.,Baker, D.,Ekiert, D.,Bhabha, G. (登録日: 2020-07-16, 公開日: 2020-12-23, 最終更新日: 2024-03-06)

主引用文献

Hsia, Y.,Mout, R.,Sheffler, W.,Edman, N.I.,Vulovic, I.,Park, Y.J.,Redler, R.L.,Bick, M.J.,Bera, A.K.,Courbet, A.,Kang, A.,Brunette, T.J.,Nattermann, U.,Tsai, E.,Saleem, A.,Chow, C.M.,Ekiert, D.,Bhabha, G.,Veesler, D.,Baker, D.
Design of multi-scale protein complexes by hierarchical building block fusion.
Nat Commun, 12:2294-2294, 2021

PubMed Abstract: A systematic and robust approach to generating complex protein nanomaterials would have broad utility. We develop a hierarchical approach to designing multi-component protein assemblies from two classes of modular building blocks: designed helical repeat proteins (DHRs) and helical bundle oligomers (HBs). We first rigidly fuse DHRs to HBs to generate a large library of oligomeric building blocks. We then generate assemblies with cyclic, dihedral, and point group symmetries from these building blocks using architecture guided rigid helical fusion with new software named WORMS. X-ray crystallography and cryo-electron microscopy characterization show that the hierarchical design approach can accurately generate a wide range of assemblies, including a 43 nm diameter icosahedral nanocage. The computational methods and building block sets described here provide a very general route to de novo designed protein nanomaterials.

PubMed: 33863889
DOI: 10.1038/s41467-021-22276-z

実験手法

ELECTRON MICROSCOPY (3.7 Å)