6XP5

Head-Middle module of Mediator

Summary for 6XP5

• Entry DOI: 10.2210/pdb6xp5/pdb
• EMDB information: 22284
• Descriptor: Mediator of RNA polymerase II transcription subunit 21, Mediator of RNA polymerase II transcription subunit 11, Mediator of RNA polymerase II transcription subunit 18, ... (15 entities in total)
• Functional Keywords: transcription
• Biological source: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719); More
• Total number of polymer chains: 15
• Total formula weight: 568879.37

Authors

Zhang, H.Q.,Chen, D.C.,Kornberg, R.D. (deposition date: 2020-07-08, release date: 2021-03-03, Last modification date: 2025-05-21)

Primary citation

Zhang, H.,Chen, D.H.,Mattoo, R.U.H.,Bushnell, D.A.,Wang, Y.,Yuan, C.,Wang, L.,Wang, C.,Davis, R.E.,Nie, Y.,Kornberg, R.D.
Mediator structure and conformation change.
Mol.Cell, 81:1781-1788.e4, 2021

PubMed Abstract: Mediator is a universal adaptor for transcription control. It serves as an interface between gene-specific activator or repressor proteins and the general RNA polymerase II (pol II) transcription machinery. Previous structural studies revealed a relatively small part of Mediator and none of the gene activator-binding regions. We have determined the cryo-EM structure of the Mediator at near-atomic resolution. The structure reveals almost all amino acid residues in ordered regions, including the major targets of activator proteins, the Tail module, and the Med1 subunit of the Middle module. Comparison of Mediator structures with and without pol II reveals conformational changes that propagate across the entire Mediator, from Head to Tail, coupling activator- and pol II-interacting regions.

PubMed: 33571424
DOI: 10.1016/j.molcel.2021.01.022

Experimental method

ELECTRON MICROSCOPY (4.2 Å)