6XO4

CryoEM structure of Eastern Equine Encephalitis (EEEV) VLP

6XO4 の概要

• エントリーDOI: 10.2210/pdb6xo4/pdb
• EMDBエントリー: 22276 22277
• 分子名称: Togavirin (3 entities in total)
• 機能のキーワード: vlp, eeev, viral protein, virus
• 由来する生物種: Eastern equine encephalitis virus (EEEV); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 496808.64

構造登録者

Binshtein, E.,Crowe, J.E. (登録日: 2020-07-06, 公開日: 2020-12-23, 最終更新日: 2024-10-23)

主引用文献

Williamson, L.E.,Gilliland Jr., T.,Yadav, P.K.,Binshtein, E.,Bombardi, R.,Kose, N.,Nargi, R.S.,Sutton, R.E.,Durie, C.L.,Armstrong, E.,Carnahan, R.H.,Walker, L.M.,Kim, A.S.,Fox, J.M.,Diamond, M.S.,Ohi, M.D.,Klimstra, W.B.,Crowe Jr., J.E.
Human Antibodies Protect against Aerosolized Eastern Equine Encephalitis Virus Infection.
Cell, 183:1884-1900.e23, 2020

PubMed Abstract: Eastern equine encephalitis virus (EEEV) is one of the most virulent viruses endemic to North America. No licensed vaccines or antiviral therapeutics are available to combat this infection, which has recently shown an increase in human cases. Here, we characterize human monoclonal antibodies (mAbs) isolated from a survivor of natural EEEV infection with potent (<20 pM) inhibitory activity of EEEV. Cryo-electron microscopy reconstructions of two highly neutralizing mAbs, EEEV-33 and EEEV-143, were solved in complex with chimeric Sindbis/EEEV virions to 7.2 Å and 8.3 Å, respectively. The mAbs recognize two distinct antigenic sites that are critical for inhibiting viral entry into cells. EEEV-33 and EEEV-143 protect against disease following stringent lethal aerosol challenge of mice with highly pathogenic EEEV. These studies provide insight into the molecular basis for the neutralizing human antibody response against EEEV and can facilitate development of vaccines and candidate antibody therapeutics.

PubMed: 33301709
DOI: 10.1016/j.cell.2020.11.011

実験手法

ELECTRON MICROSCOPY (4.2 Å)