6XLN

Cryo-EM structure of E. coli RNAP-DNA elongation complex 2 (RDe2) in EcmrR-dependent transcription

6XLN の概要

• エントリーDOI: 10.2210/pdb6xln/pdb
• EMDBエントリー: 22234 22235 22236 22237 22245 22246 22247 22248 22249
• 分子名称: DNA-directed RNA polymerase subunit alpha, ZINC ION, DNA-directed RNA polymerase subunit beta, ... (10 entities in total)
• 機能のキーワード: transcriptional factor, transcription, transferase-dna complex, promoter escape, multidrug recognition, transferase/dna
• 由来する生物種: Escherichia coli O157:H7; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 427127.49

構造登録者

Yang, Y.,Liu, C.,Liu, B. (登録日: 2020-06-28, 公開日: 2021-04-07, 最終更新日: 2024-03-06)

主引用文献

Yang, Y.,Liu, C.,Zhou, W.,Shi, W.,Chen, M.,Zhang, B.,Schatz, D.G.,Hu, Y.,Liu, B.
Structural visualization of transcription activated by a multidrug-sensing MerR family regulator.
Nat Commun, 12:2702-2702, 2021

PubMed Abstract: Bacterial RNA polymerase (RNAP) holoenzyme initiates transcription by recognizing the conserved -35 and -10 promoter elements that are optimally separated by a 17-bp spacer. The MerR family of transcriptional regulators activate suboptimal 19-20 bp spacer promoters in response to myriad cellular signals, ranging from heavy metals to drug-like compounds. The regulation of transcription by MerR family regulators is not fully understood. Here we report one crystal structure of a multidrug-sensing MerR family regulator EcmrR and nine cryo-electron microscopy structures that capture the EcmrR-dependent transcription process from promoter opening to initial transcription to RNA elongation. These structures reveal that EcmrR is a dual ligand-binding factor that reshapes the suboptimal 19-bp spacer DNA to enable optimal promoter recognition, sustains promoter remodeling to stabilize initial transcribing complexes, and finally dissociates from the promoter to reverse DNA remodeling and facilitate the transition to elongation. Our findings yield a comprehensive model for transcription regulation by MerR family factors and provide insights into the transition from transcription initiation to elongation.

PubMed: 33976201
DOI: 10.1038/s41467-021-22990-8

実験手法

ELECTRON MICROSCOPY (2.8 Å)