6XI8

Yeast TFIIK (Kin28/Ccl1/Tfb3) Complex

Summary for 6XI8

• Entry DOI: 10.2210/pdb6xi8/pdb
• EMDB information: 22191
• Descriptor: RNA polymerase II transcription factor B subunit 3, Serine/threonine-protein kinase KIN28, Cyclin CCL1, ... (5 entities in total)
• Functional Keywords: polymerase ctd, tfiih, phosphorylation, kinase, cdk, cyclin, transciption, transciption-transferase complex, transciption/transferase
• Biological source: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); More
• Total number of polymer chains: 3
• Total formula weight: 79979.97

Authors

van Eeuwen, T.,Murakami, K.,Li, T.,Tsai, K.L. (deposition date: 2020-06-19, release date: 2021-04-28)

Primary citation

van Eeuwen, T.,Li, T.,Kim, H.J.,Gorbea Colon, J.J.,Parker, M.I.,Dunbrack, R.L.,Garcia, B.A.,Tsai, K.L.,Murakami, K.
Structure of TFIIK for phosphorylation of CTD of RNA polymerase II.
Sci Adv, 7:-, 2021

PubMed Abstract: During transcription initiation, the general transcription factor TFIIH marks RNA polymerase II by phosphorylating Ser5 of the carboxyl-terminal domain (CTD) of Rpb1, which is followed by extensive modifications coupled to transcription elongation, mRNA processing, and histone dynamics. We have determined a 3.5-Å resolution cryo-electron microscopy (cryo-EM) structure of the TFIIH kinase module (TFIIK in yeast), which is composed of Kin28, Ccl1, and Tfb3, yeast homologs of CDK7, cyclin H, and MAT1, respectively. The carboxyl-terminal region of Tfb3 was lying at the edge of catalytic cleft of Kin28, where a conserved Tfb3 helix served to stabilize the activation loop in its active conformation. By combining the structure of TFIIK with the previous cryo-EM structure of the preinitiation complex, we extend the previously proposed model of the CTD path to the active site of TFIIK.

PubMed: 33827808
DOI: 10.1126/sciadv.abd4420

Experimental method

ELECTRON MICROSCOPY (3.64 Å)