6XFJ

Crystal structure of the type III secretion pilotin InvH

Summary for 6XFJ

• Entry DOI: 10.2210/pdb6xfj/pdb
• Descriptor: Type 3 secretion system pilotin, CADMIUM ION, CHLORIDE ION, ... (5 entities in total)
• Functional Keywords: pilotin, chaperone, type iii secretion, dimer, protein transport
• Biological source: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
• Total number of polymer chains: 4
• Total formula weight: 40274.33

Authors

Majewski, D.D.,Okon, M.,Heinkel, F.,Robb, C.S.,Vuckovic, M.,McIntosh, L.P.,Strynadka, N.C.J. (deposition date: 2020-06-15, release date: 2020-09-16, Last modification date: 2024-03-06)

Primary citation

Majewski, D.D.,Okon, M.,Heinkel, F.,Robb, C.S.,Vuckovic, M.,McIntosh, L.P.,Strynadka, N.C.J.
Characterization of the Pilotin-Secretin Complex from the Salmonella enterica Type III Secretion System Using Hybrid Structural Methods.
Structure, 29:125-138.e5, 2021

PubMed Abstract: The type III secretion system (T3SS) is a multi-membrane-spanning protein channel used by Gram-negative pathogenic bacteria to secrete effectors directly into the host cell cytoplasm. In the many species reliant on the T3SS for pathogenicity, proper assembly of the outer membrane secretin pore depends on a diverse family of lipoproteins called pilotins. We present structural and biochemical data on the Salmonella enterica pilotin InvH and the S domain of its cognate secretin InvG. Characterization of InvH by X-ray crystallography revealed a dimerized, α-helical pilotin. Size-exclusion-coupled multi-angle light scattering and small-angle X-ray scattering provide supporting evidence for the formation of an InvH homodimer in solution. Structures of the InvH-InvG heterodimeric complex determined by X-ray crystallography and NMR spectroscopy indicate a predominantly hydrophobic interface. Knowledge of the interaction between InvH and InvG brings us closer to understanding the mechanisms by which pilotins assemble the secretin pore.

PubMed: 32877645
DOI: 10.1016/j.str.2020.08.006

Experimental method

X-RAY DIFFRACTION (1.2 Å)