6XF2

Nesprin-1G (aa2070-2200)-FHOD1(aa1-339) complex, H. sapiens

6XF2 の概要

• エントリーDOI: 10.2210/pdb6xf2/pdb
• 関連するPDBエントリー: 6XF1
• 分子名称: Nesprin-1, FH1/FH2 domain-containing protein 1 (2 entities in total)
• 機能のキーワード: spectrin repeat, fh3 domain, armadillo repeat, scaffold protein, nuclear positioning, transmembrane, actin-associated, nuclear line, structural protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 101497.79

構造登録者

Lim, S.M.,Schwartz, T.U. (登録日: 2020-06-15, 公開日: 2021-02-03, 最終更新日: 2023-10-18)

主引用文献

Lim, S.M.,Cruz, V.E.,Antoku, S.,Gundersen, G.G.,Schwartz, T.U.
Structures of FHOD1-Nesprin1/2 complexes reveal alternate binding modes for the FH3 domain of formins.
Structure, 29:540-552.e5, 2021

PubMed Abstract: The nuclear position in eukaryotes is controlled by a nucleo-cytoskeletal network, critical in cell differentiation, division, and movement. Forces are transmitted through conserved Linker of Nucleoskeleton and Cytoskeleton (LINC) complexes that traverse the nuclear envelope and engage on either side of the membrane with diverse binding partners. Nesprin-2-giant (Nes2G), a LINC element in the outer nuclear membrane, connects to the actin directly as well as through FHOD1, a formin primarily involved in actin bundling. Here, we report the crystal structure of Nes2G bound to FHOD1 and show that the presumed G-binding domain of FHOD1 is rather a spectrin repeat (SR) binding enhancer for the neighboring FH3 domain. The structure reveals that SR binding by FHOD1 is likely not regulated by the diaphanous-autoregulatory domain helix of FHOD1. Finally, we establish that Nes1G also has one FHOD1 binding SR, indicating that these abundant, giant Nesprins have overlapping functions in actin-bundle recruitment for nuclear movement.

PubMed: 33472039
DOI: 10.1016/j.str.2020.12.013

実験手法

X-RAY DIFFRACTION (7.11 Å)