6X79

Prefusion SARS-CoV-2 S ectodomain trimer covalently stabilized in the closed conformation

6X79 の概要

• エントリーDOI: 10.2210/pdb6x79/pdb
• EMDBエントリー: 22083
• 分子名称: Spike glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: sars-cov-2, covid-19, coronavirus spike glycoprotein, fusion protein, structural genomics, seattle structural genomics center for infectious disease, ssgcid, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 435730.24

構造登録者

McCallum, M.,Walls, A.C.,Corti, D.,Veesler, D.,Seattle Structural Genomics Center for Infectious Disease (SSGCID) (登録日: 2020-05-29, 公開日: 2020-08-19, 最終更新日: 2024-11-13)

主引用文献

McCallum, M.,Walls, A.C.,Bowen, J.E.,Corti, D.,Veesler, D.
Structure-guided covalent stabilization of coronavirus spike glycoprotein trimers in the closed conformation.
Nat.Struct.Mol.Biol., 27:942-949, 2020

PubMed Abstract: SARS-CoV-2 is the causative agent of the COVID-19 pandemic, with 10 million infections and more than 500,000 fatalities by June 2020. To initiate infection, the SARS-CoV-2 spike (S) glycoprotein promotes attachment to the host cell surface and fusion of the viral and host membranes. Prefusion SARS-CoV-2 S is the main target of neutralizing antibodies and the focus of vaccine design. However, its limited stability and conformational dynamics are limiting factors for developing countermeasures against this virus. We report here the design of a construct corresponding to the prefusion SARS-CoV-2 S ectodomain trimer, covalently stabilized by a disulfide bond in the closed conformation. Structural and antigenicity analyses show we successfully shut S in the closed state without otherwise altering its architecture. We demonstrate that this strategy is applicable to other β-coronaviruses, such as SARS-CoV and MERS-CoV, and might become an important tool for structural biology, serology, vaccine design and immunology studies.

PubMed: 32753755
DOI: 10.1038/s41594-020-0483-8

実験手法

ELECTRON MICROSCOPY (2.9 Å)