6X06

Nup120 (aa1-757) from S. cerevisiae bound by VHH-SAN11

6X06 の概要

• エントリーDOI: 10.2210/pdb6x06/pdb
• 分子名称: Nucleoporin NUP120, VHH-SAN11 (2 entities in total)
• 機能のキーワード: nucleoporin, nanobody, structural protein
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 101120.85

構造登録者

Knockenhauer, K.E.,Nordeen, S.A.,Schwartz, T.U. (登録日: 2020-05-15, 公開日: 2020-12-09, 最終更新日: 2024-10-23)

主引用文献

Nordeen, S.A.,Andersen, K.R.,Knockenhauer, K.E.,Ingram, J.R.,Ploegh, H.L.,Schwartz, T.U.
A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure.
Nat Commun, 11:6179-6179, 2020

PubMed Abstract: Nuclear pore complexes (NPCs) are the main conduits for molecular exchange across the nuclear envelope. The NPC is a modular assembly of ~500 individual proteins, called nucleoporins or nups. Most scaffolding nups are organized in two multimeric subcomplexes, the Nup84 or Y complex and the Nic96 or inner ring complex. Working in S. cerevisiae, and to study the assembly of these two essential subcomplexes, we here develop a set of twelve nanobodies that recognize seven constituent nucleoporins of the Y and Nic96 complexes. These nanobodies all bind specifically and with high affinity. We present structures of several nup-nanobody complexes, revealing their binding sites. Additionally, constitutive expression of the nanobody suite in S. cerevisiae detect accessible and obstructed surfaces of the Y complex and Nic96 within the NPC. Overall, this suite of nanobodies provides a unique and versatile toolkit for the study of the NPC.

PubMed: 33268786
DOI: 10.1038/s41467-020-19884-6

実験手法

X-RAY DIFFRACTION (4.27 Å)