6X04

Nup133 (aa55-481) from S. cerevisiae bound by VHH-SAN5

6X04 の概要

• エントリーDOI: 10.2210/pdb6x04/pdb
• 分子名称: Nucleoporin NUP133, VHH-SAN5 (3 entities in total)
• 機能のキーワード: structural protein, nucleoporin, nanobody
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 367488.14

構造登録者

Nordeen, S.A.,Schwartz, T.U. (登録日: 2020-05-15, 公開日: 2020-12-09, 最終更新日: 2023-10-18)

主引用文献

Nordeen, S.A.,Turman, D.L.,Schwartz, T.U.
Yeast Nup84-Nup133 complex structure details flexibility and reveals conservation of the membrane anchoring ALPS motif.
Nat Commun, 11:6060-6060, 2020

PubMed Abstract: The hallmark of the eukaryotic cell is the complex endomembrane system that compartmentalizes cellular functions. Transport into and out of the nucleus occurs through the nuclear pore complex (NPC). The heptameric Nup84 or Y complex is an essential scaffolding component of the NPC. Here we report two nanobody-bound structures: the full-length Nup84-Nup133 C-terminal domain complex and the Nup133 N-terminal domain, both from S. cerevisiae. Together with previously published structures, this work enables the structural description of the entire 575 kDa Y complex from one species. The structure of Nup84-Nup133 details the high flexibility of this dimeric unit of the Y complex. Further, the Nup133 contains a structurally conserved amphipathic lipid packing sensor motif, confirmed by liposome interaction studies. The presented structures reveal important details about the function of the Y complex that affect our understanding of NPC structure and assembly.

PubMed: 33247142
DOI: 10.1038/s41467-020-19885-5

実験手法

X-RAY DIFFRACTION (2.68 Å)