6WTZ

Cryo-EM structure of E. Coli OmpF

6WTZ の概要

• エントリーDOI: 10.2210/pdb6wtz/pdb
• EMDBエントリー: 21900
• 分子名称: Outer membrane porin F (2 entities in total)
• 機能のキーワード: outer membrane porin, omp, ompf, membrane protein
• 由来する生物種: Escherichia coli (strain K12)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 118095.13

構造登録者

Morgan, C.E.,Su, C.-C.,Lyu, M.,Yu, E.W. (登録日: 2020-05-04, 公開日: 2021-01-20, 最終更新日: 2025-05-14)

主引用文献

Su, C.C.,Lyu, M.,Morgan, C.E.,Bolla, J.R.,Robinson, C.V.,Yu, E.W.
A 'Build and Retrieve' methodology to simultaneously solve cryo-EM structures of membrane proteins.
Nat.Methods, 18:69-75, 2021

PubMed Abstract: Single-particle cryo-electron microscopy (cryo-EM) has become a powerful technique in the field of structural biology. However, the inability to reliably produce pure, homogeneous membrane protein samples hampers the progress of their structural determination. Here, we develop a bottom-up iterative method, Build and Retrieve (BaR), that enables the identification and determination of cryo-EM structures of a variety of inner and outer membrane proteins, including membrane protein complexes of different sizes and dimensions, from a heterogeneous, impure protein sample. We also use the BaR methodology to elucidate structural information from Escherichia coli K12 crude membrane and raw lysate. The findings demonstrate that it is possible to solve high-resolution structures of a number of relatively small (<100 kDa) and less abundant (<10%) unidentified membrane proteins within a single, heterogeneous sample. Importantly, these results highlight the potential of cryo-EM for systems structural proteomics.

PubMed: 33408407
DOI: 10.1038/s41592-020-01021-2

実験手法

ELECTRON MICROSCOPY (3.15 Å)