6WOO
CryoEM structure of yeast 80S ribosome with Met-tRNAiMet, eIF5B, and GDP
This is a non-PDB format compatible entry.
Summary for 6WOO
| Entry DOI | 10.2210/pdb6woo/pdb |
| EMDB information | 21859 |
| Descriptor | 25S ribosomal RNA, eL8, uL6, ... (88 entities in total) |
| Functional Keywords | yeast, ribosome, initiation, eif5b, gdp, met-trnaimet |
| Biological source | Saccharomyces cerevisiae (Baker's yeast) More |
| Total number of polymer chains | 84 |
| Total formula weight | 3121300.62 |
| Authors | Wang, J.,Wang, J.,Puglisi, J.,Fernandez, I.S. (deposition date: 2020-04-25, release date: 2020-09-23, Last modification date: 2024-11-13) |
| Primary citation | Wang, J.,Wang, J.,Shin, B.S.,Kim, J.R.,Dever, T.E.,Puglisi, J.D.,Fernandez, I.S. Structural basis for the transition from translation initiation to elongation by an 80S-eIF5B complex. Nat Commun, 11:5003-5003, 2020 Cited by PubMed Abstract: Recognition of a start codon by the initiator aminoacyl-tRNA determines the reading frame of messenger RNA (mRNA) translation by the ribosome. In eukaryotes, the GTPase eIF5B collaborates in the correct positioning of the initiator Met-tRNA on the ribosome in the later stages of translation initiation, gating entrance into elongation. Leveraging the long residence time of eIF5B on the ribosome recently identified by single-molecule fluorescence measurements, we determine the cryoEM structure of the naturally long-lived ribosome complex with eIF5B and Met-tRNA immediately before transition into elongation. The structure uncovers an unexpected, eukaryotic specific and dynamic fidelity checkpoint implemented by eIF5B in concert with components of the large ribosomal subunit. PubMed: 33024099DOI: 10.1038/s41467-020-18829-3 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.9 Å) |
Structure validation
Download full validation report
