6WMF

Human Sun2-KASH5 complex

6WMF の概要

• エントリーDOI: 10.2210/pdb6wmf/pdb
• 分子名称: SUN domain-containing protein 2, Protein KASH5, POTASSIUM ION, ... (4 entities in total)
• 機能のキーワード: linc complex, structural protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 25158.23

構造登録者

Cruz, V.E.,Schwartz, T.U. (登録日: 2020-04-21, 公開日: 2020-12-02, 最終更新日: 2023-10-18)

主引用文献

Cruz, V.E.,Esra Demircioglu, F.,Schwartz, T.U.
Structural Analysis of Different LINC Complexes Reveals Distinct Binding Modes.
J.Mol.Biol., 432:6028-6041, 2020

PubMed Abstract: Linker of nucleoskeleton and cytoskeleton (LINC) complexes are molecular tethers that span the nuclear envelope (NE) and physically connect the nucleus to the cytoskeleton. They transmit mechanical force across the NE in processes such as nuclear anchorage, nuclear migration, and homologous chromosome pairing during meiosis. LINC complexes are composed of KASH proteins traversing the outer nuclear membrane, and SUN proteins crossing the inner nuclear membrane. Humans have several SUN- and KASH-containing proteins, yet what governs their proper engagement is poorly understood. To investigate this question, we solved high resolution crystal structures of human SUN2 in complex with the KASH-peptides of Nesprin3, Nesprin4, and KASH5. In comparison to the published structures of SUN2-KASH1/2 we observe alternative binding modes for these KASH peptides. While the core interactions between SUN and the C-terminal residues of the KASH peptide are similar in all five complexes, the extended KASH-peptide adopts at least two different conformations. The much-improved resolution allows for a more detailed analysis of other elements critical for KASH interaction, including the KASH-lid and the cation loop, and a possible self-locked state for unbound SUN. In summary, we observe distinct differences between the examined SUN-KASH complexes. These differences may have an important role in regulating the SUN-KASH network.

PubMed: 33058875
DOI: 10.1016/j.jmb.2020.09.019

実験手法

X-RAY DIFFRACTION (2.6 Å)