6WKK
Phage G gp27 major capsid proteins and gp26 decoration proteins
Summary for 6WKK
| Entry DOI | 10.2210/pdb6wkk/pdb |
| EMDB information | 21695 21702 |
| Descriptor | Gp27 major capsid protein, Gp26 capsid decoration protein (2 entities in total) |
| Functional Keywords | phage g, major capsid protein, decoration protein, capsid, icosahedral, gp26, gp27, virus |
| Biological source | Bacillus virus G More |
| Total number of polymer chains | 24 |
| Total formula weight | 469633.42 |
| Authors | Monroe, L.,Gonzalez, B.,Jiang, W.,Kihara, D. (deposition date: 2020-04-16, release date: 2020-06-10, Last modification date: 2024-03-06) |
| Primary citation | Gonzalez, B.,Monroe, L.,Li, K.,Yan, R.,Wright, E.,Walter, T.,Kihara, D.,Weintraub, S.T.,Thomas, J.A.,Serwer, P.,Jiang, W. Phage G Structure at 6.1 angstrom Resolution, Condensed DNA, and Host Identity Revision to a Lysinibacillus. J.Mol.Biol., 432:4139-4153, 2020 Cited by PubMed Abstract: Phage G has the largest capsid and genome of any known propagated phage. Many aspects of its structure, assembly, and replication have not been elucidated. Herein, we present the dsDNA-packed and empty phage G capsid at 6.1 and 9 Å resolution, respectively, using cryo-EM for structure determination and mass spectrometry for protein identification. The major capsid protein, gp27, is identified and found to share the HK97-fold universally conserved in all previously solved dsDNA phages. Trimers of the decoration protein, gp26, sit on the 3-fold axes and are thought to enhance the interactions of the hexameric capsomeres of gp27, for other phages encoding decoration proteins. Phage G's decoration protein is longer than what has been reported in other phages, and we suspect the extra interaction surface area helps stabilize the capsid. We identified several additional capsid proteins, including a candidate for the prohead protease responsible for processing gp27. Furthermore, cryo-EM reveals a range of partially full, condensed DNA densities that appear to have no contact with capsid shell. Three analyses confirm that the phage G host is a Lysinibacillus, and not Bacillus megaterium: identity of host proteins in our mass spectrometry analyses, genome sequence of the phage G host, and host range of phage G. PubMed: 32454153DOI: 10.1016/j.jmb.2020.05.016 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (6.1 Å) |
Structure validation
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