6WHI

Cryo-electron microscopy structure of the type I-F CRISPR RNA-guided surveillance complex bound to the anti-CRISPR AcrIF9

6WHI の概要

• エントリーDOI: 10.2210/pdb6whi/pdb
• EMDBエントリー: 21516 21517
• 分子名称: CRISPR-associated protein Csy1, Type I-F CRISPR-associated protein Csy2, CRISPR-associated protein Csy3, ... (8 entities in total)
• 機能のキーワード: type i-f crispr rna-guided surveillance complex, csy complex, immune system-dna-rna complex, immune system/dna/rna
• 由来する生物種: Pseudomonas aeruginosa; 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 475499.26

構造登録者

Hirschi, M.,Santiago-Frangos, A.,Wilkinson, R.,Golden, S.M.,Wiedenheft, B.,Lander, G. (登録日: 2020-04-08, 公開日: 2020-05-13, 最終更新日: 2024-03-06)

主引用文献

Hirschi, M.,Lu, W.T.,Santiago-Frangos, A.,Wilkinson, R.,Golden, S.M.,Davidson, A.R.,Lander, G.C.,Wiedenheft, B.
AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex.
Nat Commun, 11:2730-2730, 2020

PubMed Abstract: Bacteria have evolved sophisticated adaptive immune systems, called CRISPR-Cas, that provide sequence-specific protection against phage infection. In turn, phages have evolved a broad spectrum of anti-CRISPRs that suppress these immune systems. Here we report structures of anti-CRISPR protein IF9 (AcrIF9) in complex with the type I-F CRISPR RNA-guided surveillance complex (Csy). In addition to sterically blocking the hybridization of complementary dsDNA to the CRISPR RNA, our results show that AcrIF9 binding also promotes non-sequence-specific engagement with dsDNA, potentially sequestering the complex from target DNA. These findings highlight the versatility of anti-CRISPR mechanisms utilized by phages to suppress CRISPR-mediated immune systems.

PubMed: 32483187
DOI: 10.1038/s41467-020-16512-1

実験手法

ELECTRON MICROSCOPY (4.2 Å)