6WFQ

NanR dimer-DNA hetero-complex

6WFQ の概要

• エントリーDOI: 10.2210/pdb6wfq/pdb
• EMDBエントリー: 21652
• 分子名称: HTH-type transcriptional repressor NanR, DNA (5'-D(P*GP*GP*TP*AP*TP*AP*AP*CP*AP*GP*GP*TP*AP*TP*A)-3'), DNA (5'-D(P*TP*AP*TP*AP*CP*CP*TP*GP*TP*TP*AP*TP*AP*CP*C)-3') (3 entities in total)
• 機能のキーワード: nanr dimer-dna hetero-complex, transcriptional regulator, gntr superfamily, sialic acid, neu5ac, cooperativity., gene regulation
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 68308.73

構造登録者

Hariprasad, V.,Horne, C.,Santosh, P.,Amy, H.,Emre, B.,Rachel, N.,Michael, G.,Georg, R.,Borries, D.,Renwick, D. (登録日: 2020-04-03, 公開日: 2021-03-10, 最終更新日: 2024-03-06)

主引用文献

Horne, C.R.,Venugopal, H.,Panjikar, S.,Wood, D.M.,Henrickson, A.,Brookes, E.,North, R.A.,Murphy, J.M.,Friemann, R.,Griffin, M.D.W.,Ramm, G.,Demeler, B.,Dobson, R.C.J.
Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism.
Nat Commun, 12:1988-1988, 2021

PubMed Abstract: Bacteria respond to environmental changes by inducing transcription of some genes and repressing others. Sialic acids, which coat human cell surfaces, are a nutrient source for pathogenic and commensal bacteria. The Escherichia coli GntR-type transcriptional repressor, NanR, regulates sialic acid metabolism, but the mechanism is unclear. Here, we demonstrate that three NanR dimers bind a (GGTATA)-repeat operator cooperatively and with high affinity. Single-particle cryo-electron microscopy structures reveal the DNA-binding domain is reorganized to engage DNA, while three dimers assemble in close proximity across the (GGTATA)-repeat operator. Such an interaction allows cooperative protein-protein interactions between NanR dimers via their N-terminal extensions. The effector, N-acetylneuraminate, binds NanR and attenuates the NanR-DNA interaction. The crystal structure of NanR in complex with N-acetylneuraminate reveals a domain rearrangement upon N-acetylneuraminate binding to lock NanR in a conformation that weakens DNA binding. Our data provide a molecular basis for the regulation of bacterial sialic acid metabolism.

PubMed: 33790291
DOI: 10.1038/s41467-021-22253-6

実験手法

ELECTRON MICROSCOPY (3.9 Å)