6WCJ

Asymmetric vertex of the clathrin minicoat cage

6WCJ の概要

• エントリーDOI: 10.2210/pdb6wcj/pdb
• EMDBエントリー: 21608 21609 21610 21611 21612 21613 21614 21615 21616
• 分子名称: Clathrin heavy chain 1, Clathrin light chain B (2 entities in total)
• 機能のキーワード: clathrin coated vesicle, clathrin heavy chain, clathrin light chain, clathrin cage, endocytosis
• 由来する生物種: Bos taurus (Bovine); 詳細
• タンパク質・核酸の鎖数: 15
• 化学式量合計: 1876859.18

構造登録者

Paraan, M.,Mendez, J.,Sharum, S.,Kurtin, D.,He, H.,Stagg, S. (登録日: 2020-03-30, 公開日: 2020-08-19, 最終更新日: 2024-03-06)

主引用文献

Paraan, M.,Mendez, J.,Sharum, S.,Kurtin, D.,He, H.,Stagg, S.M.
The structures of natively assembled clathrin-coated vesicles.
Sci Adv, 6:eaba8397-eaba8397, 2020

PubMed Abstract: Clathrin-coated vesicles mediate trafficking of proteins and nutrients in the cell and between organelles. Proteins included in the clathrin-coated vesicles (CCVs) category include clathrin heavy chain (CHC), clathrin light chain (CLC), and a variety of adaptor protein complexes. Much is known about the structures of the individual CCV components, but data are lacking about the structures of the fully assembled complexes together with membrane and in complex with cargo. Here, we determined the structures of natively assembled CCVs in a variety of geometries. We show that the adaptor β2 appendages crosslink adjacent CHC β-propellers and that the appendage densities are enriched in CCV hexagonal faces. We resolve how adaptor protein 2 and other associated factors in hexagonal faces form an assembly hub with an extensive web of interactions between neighboring β-propellers and propose a structural model that explains how adaptor binding can direct the formation of pentagonal and hexagonal faces.

PubMed: 32743076
DOI: 10.1126/sciadv.aba8397

実験手法

ELECTRON MICROSCOPY (6.3 Å)