6WBR
Crystal structure of AceCas9 bound with guide RNA and DNA with 5'-NNNCC-3' PAM
Summary for 6WBR
Entry DOI | 10.2210/pdb6wbr/pdb |
Descriptor | CRISPR-associated endonuclease, Csn1 family,CRISPR-associated endonuclease, Csn1 family, RNA (94-MER), DNA (30-MER), ... (4 entities in total) |
Functional Keywords | dna endonuclease, crispr-cas9, hnh, ruvc, rna binding protein, rna binding protein-dna-rna complex, rna binding protein/dna/rna |
Biological source | Acidothermus cellulolyticus (strain ATCC 43068 / 11B) More |
Total number of polymer chains | 4 |
Total formula weight | 151529.24 |
Authors | |
Primary citation | Das, A.,Hand, T.H.,Smith, C.L.,Wickline, E.,Zawrotny, M.,Li, H. The molecular basis for recognition of 5'-NNNCC-3' PAM and its methylation state by Acidothermus cellulolyticus Cas9. Nat Commun, 11:6346-6346, 2020 Cited by PubMed: 33311465DOI: 10.1038/s41467-020-20204-1 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.91 Å) |
Structure validation
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