6W9Q

Peptide-bound SARS-CoV-2 Nsp9 RNA-replicase

6W9Q の概要

• エントリーDOI: 10.2210/pdb6w9q/pdb
• 分子名称: 3C-like proteinase peptide, Non-structural protein 9 fusion, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: covid-19, sars-cov-2, nsp9, rna replicase, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV); 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14687.61

構造登録者

Littler, D.R.,Gully, B.S.,Riboldi-Tunnicliffe, A.,Rossjohn, J. (登録日: 2020-03-23, 公開日: 2020-04-08, 最終更新日: 2023-10-18)

主引用文献

Littler, D.R.,Gully, B.S.,Colson, R.N.,Rossjohn, J.
Crystal Structure of the SARS-CoV-2 Non-structural Protein 9, Nsp9.
Iscience, 23:101258-101258, 2020

PubMed Abstract: Many of the SARS-CoV-2 proteins have related counterparts across the Severe Acute Respiratory Syndrome (SARS-CoV) family. One such protein is non-structural protein 9 (Nsp9), which is thought to mediate viral replication, overall virulence, and viral genomic RNA reproduction. We sought to better characterize the SARS-CoV-2 Nsp9 and subsequently solved its X-ray crystal structure, in an apo form and, unexpectedly, in a peptide-bound form with a sequence originating from a rhinoviral 3C protease sequence (LEVL). The SARS-CoV-2 Nsp9 structure revealed the high level of structural conservation within the Nsp9 family. The exogenous peptide binding site is close to the dimer interface and impacted the relative juxtapositioning of the monomers within the homodimer. We have established a protocol for the production of SARS-CoV-2 Nsp9, determined its structure, and identified a peptide-binding site that warrants further study to understanding Nsp9 function.

PubMed: 32592996
DOI: 10.1016/j.isci.2020.101258

実験手法

X-RAY DIFFRACTION (2.05 Å)