6W32

Crystal structure of Sfh5

Summary for 6W32

• Entry DOI: 10.2210/pdb6w32/pdb
• Descriptor: Phosphatidylinositol transfer protein SFH5, PROTOPORPHYRIN IX CONTAINING FE (2 entities in total)
• Functional Keywords: signaling protein
• Biological source: Saccharomyces cerevisiae (strain YJM789) (Baker's yeast)
• Total number of polymer chains: 3
• Total formula weight: 109032.50

Authors

Gulten, G.,Khan, D.,Aggarwal, A.,Krieger, I.,Sacchettini, J.C.,Bankaitis, V.A. (deposition date: 2020-03-08, release date: 2020-11-25, Last modification date: 2024-03-06)

Primary citation

Khan, D.,Lee, D.,Gulten, G.,Aggarwal, A.,Wofford, J.,Krieger, I.,Tripathi, A.,Patrick, J.W.,Eckert, D.M.,Laganowsky, A.,Sacchettini, J.,Lindahl, P.,Bankaitis, V.A.
A Sec14-like phosphatidylinositol transfer protein paralog defines a novel class of heme-binding proteins.
Elife, 9:-, 2020

PubMed Abstract: Yeast Sfh5 is an unusual member of the Sec14-like phosphatidylinositol transfer protein (PITP) family. Whereas PITPs are defined by their abilities to transfer phosphatidylinositol between membranes in vitro, and to stimulate phosphoinositide signaling in vivo, Sfh5 does not exhibit these activities. Rather, Sfh5 is a redox-active penta-coordinate high spin Fe hemoprotein with an unusual heme-binding arrangement that involves a co-axial tyrosine/histidine coordination strategy and a complex electronic structure connecting the open shell iron -orbitals with three aromatic ring systems. That Sfh5 is not a PITP is supported by demonstrations that heme is not a readily exchangeable ligand, and that phosphatidylinositol-exchange activity is resuscitated in heme binding-deficient Sfh5 mutants. The collective data identify Sfh5 as the prototype of a new class of fungal hemoproteins, and emphasize the versatility of the Sec14-fold as scaffold for translating the binding of chemically distinct ligands to the control of diverse sets of cellular activities.

PubMed: 32780017
DOI: 10.7554/eLife.57081

Experimental method

X-RAY DIFFRACTION (2.9 Å)