6W2S

Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1)

これはPDB形式変換不可エントリーです。

6W2S の概要

• エントリーDOI: 10.2210/pdb6w2s/pdb
• EMDBエントリー: 21529
• 分子名称: 18S rRNA, uS4, uS17, ... (45 entities in total)
• 機能のキーワード: crpv 5'-utr ires, internal ribosome entry site, ribosome, ribosome-translation complex, ribosome/translation
• 由来する生物種: Cricket paralysis virus; 詳細
• タンパク質・核酸の鎖数: 43
• 化学式量合計: 1840048.79

構造登録者

Neupane, R.,Pisareva, V.,Rodriguez, C.F.,Pisarev, A.,Fernandez, I.S. (登録日: 2020-03-08, 公開日: 2020-04-22, 最終更新日: 2024-03-06)

主引用文献

Neupane, R.,Pisareva, V.P.,Rodriguez, C.F.,Pisarev, A.V.,Fernandez, I.S.
A complex IRES at the 5'-UTR of a viral mRNA assembles a functional 48S complex via an uAUG intermediate.
Elife, 9:-, 2020

PubMed Abstract: Taking control of the cellular apparatus for protein production is a requirement for virus progression. To ensure this control, diverse strategies of cellular mimicry and/or ribosome hijacking have evolved. The initiation stage of translation is especially targeted as it involves multiple steps and the engagement of numerous initiation factors. The use of structured RNA sequences, called nternal ibosomal ntry ites (IRES), in viral RNAs is a widespread strategy for the exploitation of eukaryotic initiation. Using a combination of electron cryo-microscopy (cryo-EM) and reconstituted translation initiation assays with native components, we characterized how a novel IRES at the 5'-UTR of a viral RNA assembles a functional initiation complex via an uAUG intermediate. The IRES features a novel extended, multi-domain architecture, that circles the 40S head. The structures and accompanying functional data illustrate the importance of 5'-UTR regions in translation regulation and underline the relevance of the untapped diversity of viral IRESs.

PubMed: 32286223
DOI: 10.7554/eLife.54575

実験手法

ELECTRON MICROSCOPY (3.47 Å)