6VYB

SARS-CoV-2 spike ectodomain structure (open state)

6VYB の概要

• エントリーDOI: 10.2210/pdb6vyb/pdb
• EMDBエントリー: 21452 21457
• 分子名称: Spike glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: coronavirus, sars-cov-2, sars-cov, spike glycoprotein, fusion protein, structural genomics, seattle structural genomics center for infectious disease, ssgcid, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 437436.48

構造登録者

Walls, A.C.,Park, Y.J.,Tortorici, M.A.,Wall, A.,Seattle Structural Genomics Center for Infectious Disease (SSGCID),McGuire, A.T.,Veesler, D. (登録日: 2020-02-25, 公開日: 2020-03-11, 最終更新日: 2020-07-29)

主引用文献

Walls, A.C.,Park, Y.J.,Tortorici, M.A.,Wall, A.,McGuire, A.T.,Veesler, D.
Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein.
Cell, 181:281-, 2020

PubMed Abstract: The emergence of SARS-CoV-2 has resulted in >90,000 infections and >3,000 deaths. Coronavirus spike (S) glycoproteins promote entry into cells and are the main target of antibodies. We show that SARS-CoV-2 S uses ACE2 to enter cells and that the receptor-binding domains of SARS-CoV-2 S and SARS-CoV S bind with similar affinities to human ACE2, correlating with the efficient spread of SARS-CoV-2 among humans. We found that the SARS-CoV-2 S glycoprotein harbors a furin cleavage site at the boundary between the S/S subunits, which is processed during biogenesis and sets this virus apart from SARS-CoV and SARS-related CoVs. We determined cryo-EM structures of the SARS-CoV-2 S ectodomain trimer, providing a blueprint for the design of vaccines and inhibitors of viral entry. Finally, we demonstrate that SARS-CoV S murine polyclonal antibodies potently inhibited SARS-CoV-2 S mediated entry into cells, indicating that cross-neutralizing antibodies targeting conserved S epitopes can be elicited upon vaccination.

PubMed: 32155444
DOI: 10.1016/j.cell.2020.02.058

実験手法

ELECTRON MICROSCOPY (3.2 Å)