6VY1

Cryo-EM structure of filamentous PFD from Methanocaldococcus jannaschii

6VY1 の概要

• エントリーDOI: 10.2210/pdb6vy1/pdb
• EMDBエントリー: 21455
• 分子名称: Prefoldin subunit alpha 2 (1 entity in total)
• 機能のキーワード: helical symmetry, nanowire, prefoldin, protein fibril
• 由来する生物種: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 229748.97

構造登録者

Wang, F.,Chen, Y.X.,Ing, N.L.,Hochbaum, A.I.,Clark, D.S.,Glover, D.J.,Egelman, E.H. (登録日: 2020-02-25, 公開日: 2020-05-13, 最終更新日: 2025-05-14)

主引用文献

Chen, Y.X.,Ing, N.L.,Wang, F.,Xu, D.,Sloan, N.B.,Lam, N.T.,Winter, D.L.,Egelman, E.H.,Hochbaum, A.I.,Clark, D.S.,Glover, D.J.
Structural Determination of a Filamentous Chaperone to Fabricate Electronically Conductive Metalloprotein Nanowires.
Acs Nano, 14:6559-6569, 2020

PubMed Abstract: The transfer of electrons through protein complexes is central to cellular respiration. Exploiting proteins for charge transfer in a controllable fashion has the potential to revolutionize the integration of biological systems and electronic devices. Here we characterize the structure of an ultrastable protein filament and engineer the filament subunits to create electronically conductive nanowires under aqueous conditions. Cryoelectron microscopy was used to resolve the helical structure of gamma-prefoldin, a filamentous protein from a hyperthermophilic archaeon. Conjugation of tetra-heme c3-type cytochromes along the longitudinal axis of the filament created nanowires capable of long-range electron transfer. Electrochemical transport measurements indicated networks of the nanowires capable of conducting current between electrodes at the redox potential of the cytochromes. Functionalization of these highly engineerable nanowires with other molecules, such as redox enzymes, may be useful for bioelectronic applications.

PubMed: 32347705
DOI: 10.1021/acsnano.9b09405

実験手法

ELECTRON MICROSCOPY (6 Å)